Serum concentration of soluble form ICAM-1, PECAM-1, VAP-1, E-selectin, VCAM-1 in 20 patients with Erythema migrans and 20 patients with Lyme arthritis before and after 4-weeks antibiotic treatment were estimated. Comparing group consited 8 healthy volunteers. Concentrations of soluble molecules were performed with ELISA kits(Bender MedSystem, Austria). Results of the study indicate involvment of soluble form ICAM-1, PECAM-1, VAP-1, E-selectin and VCAM-1 in immunopathogenesis of Lyme borreliosis, and different engagement of molecules in depend on stage of disease. In early localized stage of disease, results suggest participation molecules engaged in all stages of "adhesion cascade" whilst in disseminated, late stage of Lyme borreliosis molecules of tight adhesion. The measurement of soluble form of adhesion molecules can be useful of in differentiation of localized and disseminated late stages of Lyme borreliosis helpful in clinical diagnosis.